| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Hancock High School | A | Britton | Martin | Whitelow | Stewart | 1:46.74 | Verified |
| 3 | Maplewood-Richmond Hts. High Sch | A | Gavin | Carter | Gustin | Shaw | 1:35.37 | Verified |
| 4 | Brentwood High School | A | Chantharasy | Hussain | Taylor | Morgan-Scott | 1:39.60 | Verified |
| 5 | Bayless High School | A | Kelly | Patton | Malkic | Uebari | 1:49.53 | Verified |
