| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Maplewood-Richmond Hts. High Sch | A | Means | Orahood | Means | Royston | DNF | Verified |
| 2 | Hancock High School | A | Hurd | Martin | Sanders | Whitelow | 48.92 | Verified |
| 3 | Brentwood High School | A | Clay | Harris | Phillips | Lane | 46.00 | Verified |
| 4 | Bayless High School | A | Miller | Uebari | Kelly | Patton | 48.39 | Verified |
| 5 | Valley Park High School | A | Walker-Townsend | Bonnett | Williams | Sears | DQ | Verified |
