| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Maplewood-Richmond Hts. High Sch | A | Robinson | Hurn | Brown | Harper-McCain | 1:02.30 | Verified |
| 3 | Bayless High School | A | Jugovic | Ladouceur | Lormine | Dodson | 56.69 | Verified |
| 4 | Hancock High School | A | Birchfield | Alivernia | Hunter | Haynes-Mcginnist | 1:00.23 | Verified |
