| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | McKinley Classical Leadership Hi | A | Cotton | Johnson | Winfield | Bledsoe | NT | Unverified: |
| 4 | Sumner High School | A | 1:47.09 | Verified | ||||
| 5 | STEAM Academy at McCluer South-B | B | Sralla | Simpson-Johnson | Holliman | Harlan | 1:48.81 | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | STEAM Academy at McCluer South-B | A | Johnson | Jackson | Grimes | Moreland-Bell | 1:40.81 | Unverified: |
| 3 | KIPP St. Louis | A | Baker | Cook | Moore | Ray | 1:39.00 | Unverified: Lutheran North Home Opener |
| 4 | McCluer High School | A | Johnson | McCoy | Bills | Reynolds | 1:34.05 | Verified |
| 5 | Riverview Gardens High School | A | Carlock | Hodges | Howard | Love | 1:35.30 | Unverified: |
| 7 | Gateway High School | A | Bell | Calvin | Spencer | Rayner | 1:41.59 | Verified |
