| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Gateway High School | A | Bell | Shelton | Spencer | Warren | 4:14.73 | Verified |
| 4 | Riverview Gardens High School | A | Abel | Crymes | Davis | Love | 3:43.59 | Verified |
| 5 | Normandy High School | A | Johnson | Moore | Woods | Sullivan | 3:46.84 | Unverified: Rod Staggs Freshman Sophomore Me |
| 6 | STEAM Academy at McCluer South-B | A | Parker | Fields | Hearon | Kinkade-Evans | 3:54.41 | Verified |
