| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Collegiate School of Medicine an | A | Ebenhoh | Harris | Owens | Smith | 5:05.54 | Verified |
| 3 | Normandy High School | A | Griffin | Howard | Jenkins | Winston | 4:54.34 | Verified |
| 4 | STEAM Academy at McCluer South-B | A | Coleman | Thames | Reed | Thames | 4:06.83 | Unverified: |
| 5 | STEAM Academy at McCluer South-B | B | Green | Saunders | Anderson | Cromer | 4:20.83 | Verified |
| 6 | Riverview Gardens High School | A | GLADDEN | Smith | Zimmerman | Richardson | 4:59.00 | Unverified: Charlie Finch Invitational |
