| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CANNIDA, Kaliyah | SR | Gateway High School | NT | Unverified: |
| 2 | BALTIMORE, Trenice | 9 | McCluer High School | 1:08.80 | Verified |
| 3 | BROWN, Anyah | SO | McCluer High School | 1:01.03 | Verified |
| 4 | WINSTON, Donye' | SO | Normandy High School | NT | Unverified: |
| 5 | GLADDEN, Mykale | JR | Riverview Gardens High School | 59.73 | Verified |
| 6 | COOKE, Breyonna | SO | Sumner High School | 1:04.05 | Verified |
| 7 | SAUNDERS, Sa'Niya | SO | STEAM Academy at McCluer South-B | NT | Unverified: |
