| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | McKinley Classical Leadership Hi | A | Brooks | Bledsoe | Winfield | Johnson | NT | Unverified: |
| 4 | Sumner High School | A | 52.45 | Verified | ||||
| 5 | KIPP St. Louis | A | Jones | Jones | Muhammad | Welch | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | STEAM Academy at McCluer South-B | A | Grimes | Jackson | Johnson | Frenchie | 45.81 | Unverified: |
| 4 | Riverview Gardens High School | A | Carlock | Hodges | Howard | Jones | 45.00 | Unverified: |
| 5 | McCluer High School | A | Reynolds | McCoy | Jones | Mosley | 45.06 | Verified |
| 6 | Gateway High School | A | Bell | Rayner | Spencer | Thurston | 47.64 | Verified |
| 7 | STEAM Academy at McCluer South-B | B | Sralla | Simpson-Johnson | Harlan | Holliman | 50.74 | Unverified: |
