| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Collegiate School of Medicine an | A | Bohlen | Luster | Vaughn | Robinson | NT | Unverified: |
| 4 | Sumner High School | A | 1:04.88 | Verified | ||||
| 5 | McKinley Classical Leadership Hi | A | Galarza | Jones | Kamara | Kamara | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | McCluer High School | A | Townsend | House | Robinson | Pool | 56.33 | Verified |
| 2 | Riverview Gardens High School | B | 55.61 | Verified | ||||
| 3 | STEAM Academy at McCluer South-B | B | Hall | Cromer | Clark | Laney | 53.18 | Verified |
| 4 | STEAM Academy at McCluer South-B | A | Reed | Reed | Coleman | Thames | 49.88 | Unverified: |
| 6 | Gateway High School | A | Brown | Cannamore | Gray | Nevils | 55.00 | Verified |
| 7 | Riverview Gardens High School | A | Long | Richardson | harris | Tabron | 55.61 | Verified |
