| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | East St. Louis (Senior) HS | A | Baxton | Bucanan | Ferguson | Estes | NT | Unverified: |
| 4 | Riverview Gardens High School | A | Abel | Bibbs | Bowman | Jackson | NT | Unverified: |
| 5 | McCluer High School | A | Bills | Wright | Johnson | Greenlee | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Roosevelt High School | A | Collins | Milton | Lee | Scott | 1:47.89 | Verified |
| 2 | STEAM Academy at McCluer South-B | A | Coleman | Gregory | Grimes | Moreland-Bell | 1:42.00 | Unverified: |
| 3 | Normandy High School | A | Allen | Moore | Crawford | Graham | 1:38.50 | Unverified: |
| 4 | Hazelwood Central High School | A | Jones | Williams-Trice | McDaniel | Coleman | 1:34.97 | Verified |
| 5 | KIPP St. Louis | A | Baker | Muhammad | Ray | Ward | NT | Verified |
| 6 | Hazelwood West High School | A | 1:40.69 | Verified | ||||
| 7 | Belleville East High School | A | Peals | Jackson | Brown | Patton | 1:43.00 | Unverified: |
| 8 | Lift for Life Academy Charter Hi | A | Penn | Norrington | Morgan | Grissom | NT | Unverified: |
