| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Normandy High School | A | Griffin | Houston | Jenkins | Quilling | NT | Unverified: |
| 4 | Lift for Life Academy Charter Hi | A | Gillespie | Lindsey | Simmons | Fowler | NT | Unverified: |
| 5 | Metro High School | A | Fuchs | Macon-Ford | Meyer | Hollingsworth | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | McCluer High School | A | Pool | Robinson | Burr | Marshall | NT | Unverified: |
| 2 | McCluer North High School | A | Boyd | Love | Goforth | Taylor | 2:02.75 | Verified |
| 3 | Hazelwood Central High School | A | Swanson | Witherspoon | Reece | Williams | 1:52.30 | Verified |
| 4 | STEAM Academy at McCluer South-B | A | Reed | Cromer | Saunders | Green | 1:49.97 | Unverified: |
| 5 | East St. Louis (Senior) HS | A | 1:50.00 | Verified | ||||
| 6 | Hazelwood East High School | A | Wiggins | Davis | Brown | Carter | 2:00.30 | Unverified: |
| 7 | Hazelwood West High School | A | 2:05.07 | Verified | ||||
| 8 | Ritenour High School | A | Brantley | Gales | Henley | Jones | DQ | Unverified: |
