| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | STEAM Academy at McCluer South-B | A | Johnson | Hearon | Holliman | Simpson-Johnson | NT | Unverified: |
| 4 | Hazelwood Central High School | A | Bright | Lee | Anthony | Thornton | 1:34.97 | Verified |
| 5 | Belleville East High School | A | Smith | Williams | Wright | Mitchell | 1:42.00 | Unverified: |
| 6 | Cardinal Ritter High School | A | Batteast | Brooks | Anderson | Summers JR. | NT | Unverified: |
