| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Ritenour High School | A | Jones | Owens | Payne | Payne | NT | Unverified: |
| 4 | Metro High School | A | Anania | Tibbs | Clair | Leonard | NT | Unverified: |
| 5 | Normandy High School | A | Houston | Quilling | Walton | Winston | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | McCluer North High School | A | Boyd | Goforth | Taylor | Smith | 5:12.57 | Verified |
| 3 | Cardinal Ritter High School | A | NT | |||||
| 4 | East St. Louis (Senior) HS | A | 4:20.00 | Verified | ||||
| 5 | STEAM Academy at McCluer South-B | A | Saunders | Green | Anderson | Strehle | 4:20.83 | Verified |
| 6 | Hazelwood Central High School | A | Catching | Elam | Maxwell | Jones | 4:59.27 | Unverified: |
| 7 | Hazelwood West High School | A | 5:26.25 | Verified |
