| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | KIPP St. Louis | A | Baker | Jones | Jones | Jones | NT | Unverified: |
| 4 | McCluer High School | A | Middleton | Cooper | Johnson | Johnson | NT | Unverified: |
| 5 | East St. Louis (Senior) HS | A | BOWNES | McClain | ELLARD | Ryan | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | STEAM Academy at McCluer South-B | A | Harlan | Simpson-Johnson | Sralla | Hines | NT | Unverified: |
| 2 | Hazelwood Central High School | A | Davis | Pfingsten | Reed-Warren | Jarmon | NT | Unverified: |
| 3 | Cardinal Ritter High School | A | NT | |||||
| 4 | Belleville East High School | A | Fullwe | Schnarre | Taylor | Wittenuaer | 2:00.00 | Unverified: |
| 5 | Roosevelt High School | A | Smith | Williams | Lee | Murphy | NT | Unverified: |
| 6 | Hazelwood West High School | A | NT | Unverified: | ||||
| 7 | Metro High School | A | Badillo | Penny-Johnson | Keoshkerian | Cade | NT | Unverified: |
| 8 | Oakville High School | A | Williams | Edwards | BLICK | GOLD | NT | Unverified: |
