| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | KIPP St. Louis | A | NT | |||||
| 3 | Metro High School | A | Fuchs | Macon-Ford | Upadhya | Green | NT | Unverified: |
| 4 | Hazelwood West High School | A | NT | Unverified: | ||||
| 5 | Hazelwood Central High School | A | Love | Witherspoon | Reece | Catching | NT | Unverified: |
| 6 | McCluer High School | A | House | Townsend | Hooks | Marshall | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Roosevelt High School | A | Glover | Robinson | Phillips | Mayhew | NT | Unverified: |
| 2 | Normandy High School | A | Brownlee | Griffin | Houston | Quilling | NT | Unverified: |
| 3 | McCluer North High School | A | Love | Boyd | Beck | Taylor | NT | Unverified: |
| 4 | East St. Louis (Senior) HS | A | 1:50.00 | Unverified: 1:50.00 | ||||
| 5 | STEAM Academy at McCluer South-B | A | Cromer | Anderson | Clark | Hall | 2:04.90 | Unverified: |
| 6 | Cardinal Ritter High School | A | NT | |||||
| 7 | Hazelwood East High School | A | Brown | Davis | Strong | Carter | NT | Unverified: |
| 8 | Ritenour High School | A | Payne | Brantley | Gales | Payne | NT | Unverified: |
