| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Metro High School | A | Badillo | Long | Baum-Scholle | Gonzalez | NT | Unverified: |
| 2 | East St. Louis (Senior) HS | A | Baxton | Brown | Monroe | Muex | NT | Unverified: |
| 3 | Lift for Life Academy Charter Hi | A | Walker | Grissom | Norrington | Landers | NT | Unverified: |
| 4 | STEAM Academy at McCluer South-B | A | Barnett | Johnson | Evans | Parker | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 5 | Belleville East High School | A | Taylor | Cooley | Wittenuaer | Schnarre | 4:35.00 | Unverified: |
| 6 | Hazelwood Central High School | A | Jackson | Jefferson | Lee | Green | NT | Unverified: |
| 7 | McCluer North High School | A | Coleman | Davis | Rimmer | Thompson | NT | Unverified: |
| 8 | Hazelwood West High School | A | NT | Unverified: | ||||
| 9 | Westminster Christian Academy | A | Heard | Lowry | Cook | Gorline | NT | Unverified: |
