| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Hazelwood Central High School | A | Mayes | Love | Moore | Wooten | NT | Unverified: |
| 2 | McCluer North High School | A | McMiller | McBride | Minor | Watkins | NT | Unverified: |
| 3 | Hazelwood West High School | A | NT | Unverified: | ||||
| 4 | STEAM Academy at McCluer South-B | A | Reed | Cromer | Green | Saunders | 4:28.00 | Unverified: |
| 5 | East St. Louis (Senior) HS | A | 5:10.00 | Unverified: | ||||
| 6 | Metro High School | A | Upadhya | Green | Anania | Tibbs | NT | Unverified: |
| 7 | Lift for Life Academy Charter Hi | A | Gillespie | Brown | Fowler | Seals | NT | Unverified: |
