| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Hazelwood West High School | A | 58.58 | Verified | ||||
| 2 | Hazelwood Central High School | A | Swanson | Witherspoon | Reece | Williams | 58.26 | Unverified: |
| 3 | Hazelwood East High School | A | Easley | Fitch | Knox | Brown | 56.72 | Verified |
| 4 | East St. Louis (Senior) HS | A | 50.00 | Verified | ||||
| 5 | STEAM Academy at McCluer South-B | A | Reed | Clark | Hall | Burr | NT | Verified |
| 6 | McCluer North High School | A | Love | Goforth | Beck | Taylor | 56.90 | Verified |
| 7 | Metro High School | A | Green | Macon-Ford | Fuchs | Hollingsworth | NT | Verified |
| 8 | McCluer High School | A | Hooks | Townsend | Tate | Sykes | NT | Unverified: |
