| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | STEAM Academy at McCluer South-B | A | Frenchie | Sralla | Harlan | Simpson-Johnson | 49.74 | Verified |
| 4 | Belleville East High School | A | Smith | Young | Jones | Williams | 44.60 | Unverified: |
| 5 | Hazelwood Central High School | A | Atkins | Anthony | Thornton | Bright | 44.89 | Verified |
| 6 | Cardinal Ritter High School | A | Abdullah | Carter | Vonner | Rose | NT | Unverified: |
