| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Battle High School | A | Johnson | Cross | Adam | Love | NT | Unverified: |
| 4 | Rock Bridge High School | A | Bailey | Baehr | Chinikoski | Rychnovsky | 3:55.00 | Unverified: |
| 5 | Hickman High School | A | Hogan | Holt | McNally | Mariano-Sanchez | NT | Unverified: |
| 6 | Battle High School | B | Green | Carter | Lakes | Rice | NT | Unverified: |
