| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Marion County MS | A | Tuley | Stephens | Stephens | Wright | 4:54.53 | Verified |
| 4 | Palmyra MS | A | Jurado | Kroeger | McNeff | White | 4:32.44 | Verified |
| 5 | Clark County MS RED B | B | Webster | Wilson | Schmitz | Schaller | 4:38.00 | Unverified: |
| 6 | Monroe City Middle School | A | Lehenbauer | Shuck | Richardson | Mayfield | 5:19.06 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | North Shelby MS | A | Browning | Prange | Aldridge | Bock | 4:29.30 | Verified |
| 2 | South Shelby Middle School | B | Darling | McElhoe | Seaton | Kuntz | 4:28.00 | Unverified: Westran MS Relays |
| 3 | Bowling Green Middle School | A | Fairchild | Matlock | Horton | Pasley | 4:26.00 | Verified |
| 4 | Clark County MS RED | A | Hunziker | Fuller | Binsbacher | Davis | 4:10.51 | Unverified: |
| 5 | South Shelby Middle School | A | Gill | Mason | Mason | Beasley | 4:16.87 | Verified |
| 6 | Highland MS | A | Bartz | Harshberger | Post | Caudill | 4:26.84 | Verified |
| 7 | North Shelby MS | B | Barrick | Belt | Christine | Robbins | 4:29.30 | Verified |
| 8 | Clark County MS RED B | A | King | Martin | Missen | Waples | 4:32.00 | Unverified: |
