| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Clark County MS RED B | A | Filz | Schmitz | Dent | Kirchner | 1:02.60 | Unverified: |
| 4 | North Shelby MS | B | Parsons | McCoy | Bacon | Wiedeman | 1:01.69 | Verified |
| 5 | North Shelby MS | A | Daniel | Flowers | Robbins | Sharpe | 1:01.84 | Verified |
| 6 | South Shelby Middle School | B | Threlkeld | Larrick | Maubach | Strayhorn | 1:22.00 | Unverified: Brookfield MS Relays |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Marion County MS | A | Begley | Swisher | Beaver | Wright | 1:00.79 | Verified |
| 2 | South Shelby Middle School | A | McWilliams | Schmitz | Mason | Mason | 56.15 | Verified |
| 3 | Canton MS | A | Logsdon | O'Brien | Shuman | Oliver | 52.54 | Verified |
| 4 | Highland MS | A | Harshberger | Gaus | Post | Seals | 51.53 | Verified |
| 5 | Monroe City Middle School | A | Meyer | Sapp | Mayfield | Jones | 52.33 | Unverified: Highland Cougar Relays |
| 6 | Palmyra MS | A | Harper | Smyser | Snow | Spicknall | 53.72 | Verified |
| 7 | Clark County MS RED | A | Roach | Rodgers | Sammons | Weaver | 57.36 | Verified |
| 8 | Bowling Green Middle School | A | Buffington | Dyer | Splain | Horton | 1:01.05 | Verified |
