| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Union Middle School | A | Sulschi | Frazier | Burkhart | Green | 4:18.34 | Verified |
| 3 | Sullivan Middle School | A | Dudley | Harmon | Ray | Pritchett | 4:15.73 | Unverified: |
| 4 | St. Clair Junior High School | A | Bartz | Eddy | Pittman | Bradshaw | 4:05.85 | Verified |
| 5 | Owensville Middle School | A | Wellman | Marquez | Hamann | Lenauer | 4:06.54 | Verified |
| 6 | Washington Middle School | A | Brant | Elbert | Riley | Mitchem | 4:17.10 | Verified |
