| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Pacific Middle School | A | Brinson | Armistead | Short | Dattoli | 5:04.85 | Verified |
| 4 | Washington Middle School | A | Bunch-Gregory | Wang | Konefal | Compton | 4:53.69 | Verified |
| 5 | Sullivan Middle School | A | Harbour | Lambert | Denney | Denney | 4:58.73 | Verified |
| 6 | Union Middle School | A | Hilkerbaumer | O'Rourke | Nappier | Underwood | 5:08.10 | Verified |
