| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | DRYER, Grace | 8 | Pacific Middle School | 2:40.00 | Verified |
| 2 | KEISER, Josie | 8 | Washington Middle School | 2:40.60 | Verified |
| 3 | UTHLAUT, Elaina | 8 | Hermann Middle School | 2:45.63 | Verified |
| 4 | JOHANSON, Viola | 8 | Union Middle School | 2:46.94 | Verified |
| 5 | GARGRAVE, Grace | 8 | Washington Middle School | 2:53.32 | Verified |
| 6 | BREEDLOVE, Jenna | 8 | Owensville Middle School | 2:58.56 | Verified |
| 7 | SCHRECK, Cordelia | 8 | Union Middle School | 2:59.90 | Verified |
| 8 | LANE, Kealie | 8 | Hermann Middle School | 3:00.73 | Verified |
| 9 | HINSON, Lila | 7 | St. Clair Junior High School | 3:01.45 | Verified |
| 10 | WINCHESTER, Sydney | 8 | Washington Middle School | 3:01.50 | Verified |
| 11 | HEANEY, Alaina | 8 | Hermann Middle School | 3:11.89 | Verified |
| 12 | GRIFFITH, Riley | 8 | Sullivan Middle School | 3:17.68 | Verified |
| 13 | SCHATZL, Alexis | 8 | Sullivan Middle School | 3:22.22 | Verified |
| 14 | TUNE, Chloe | 8 | Owensville Middle School | 3:42.70 | Verified |
| 15 | KITCHEN, Joellie | 8 | St. Clair Junior High School | 3:45.14 | Verified |
| 16 | ADKISON-FARMER, Ella | 8 | Owensville Middle School | 4:17.60 | Verified |
| 17 | BAY, Emma | 8 | St. Clair Junior High School | NT | Unverified: |
| 18 | WALL, Sawyer | 8 | Pacific Middle School | NT | Unverified: |
