| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | St. Clair Junior High School | A | Shelton | Conner | Snell | Draves | 1:00.14 | Verified |
| 2 | Hermann Middle School | A | Fredrick | Schutt | Kahler | Hughes | 58.40 | Verified |
| 3 | Pacific Middle School | A | Douglas | Lay | Huerta | Cronovich | 57.35 | Verified |
| 4 | Sullivan Middle School | A | Carpenter | Miller | Peterson | Hulsey | 57.02 | Unverified: |
| 5 | Washington Middle School | A | McGee | Bass | Sneed | Highley | 57.18 | Verified |
| 6 | Union Middle School | A | Broeker | Sherwood | Spiller | Kliethermes | 58.36 | Verified |
| 7 | Owensville Middle School | A | Barker | Carpenter | Engelbrect | Reed | 58.51 | Verified |
