| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | St. Clair Junior High School | A | Wright | Richards | Love | Davis | 1:41.34 | Unverified: St. James |
| 3 | Pacific Middle School | A | Cox | Riegel | Hedge | Broser | 53.72 | Verified |
| 4 | Washington Middle School | A | Busch | Bast | Brooman | Mueller | 50.84 | Verified |
| 5 | Union Middle School | A | Beckmann | Gillespie | Manhart | Prilwetz | 51.28 | Verified |
| 6 | Sullivan Middle School | A | Carnahan | Gassner | Herron | Ray | 56.43 | Verified |
