| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Hermann Middle School | A | Walker | Limberopoulos | Baguio | Coon | 2:11.88 | Verified |
| 3 | Union Middle School | A | Broeker | Sherwood | Spiller | Kliethermes | 2:05.23 | Verified |
| 4 | Washington Middle School | A | Kitchen | Bass | Sneed | Hill | 1:59.62 | Verified |
| 5 | Pacific Middle School | A | Lay | Douglas | Huerta | Cronovich | 2:04.06 | Verified |
| 6 | St. Clair Junior High School | A | Shelton | Talleur | Snell | Draves | 2:07.13 | Verified |
| 7 | Owensville Middle School | A | Barker | Ryerson | McDaniel | Reed | 2:12.10 | Verified |
