| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | KIPP St. Louis | A | Davis | Smith | Ward | Wiley | 55.50 | Verified |
| 2 | McKinley Classical Leadership Hi | A | Flenoid | Johnson | Winfield | Rockett | 51.34 | Verified |
| 3 | Sumner High School | A | Abdi | Blair | Caldwell | Lacy | 48.38 | Verified |
| 4 | Roosevelt High School | A | Smith | Murphy | Bunting | Campbell | 48.54 | Verified |
| 5 | Metro High School | A | Flenoid | Furman | Green | Fisher | 51.90 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Gateway High School | A | Calvin | Rayner | Bell | Warren | 47.58 | Verified |
| 2 | Vashon High School | A | Smith | Phillips | Chandler | Hill | 46.37 | Verified |
| 3 | Lift for Life Academy Charter Hi | A | Norrington | Penn | Morgan | Owens | 45.63 | Verified |
| 4 | Soldan International Studies Hig | A | Washington | Clemons | Johnson | Nebsit | 45.88 | Verified |
| 5 | Miller Career Academy | A | Williams | Forte | Slayton | Gleghorn | 47.13 | Verified |
| 6 | Carnahan High School | A | Holmes | Long | Mull | Hester | 47.80 | Verified |
