| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | KIPP St. Louis | A | Jones | Jones | Jones | Muhammad | 55.50 | Verified |
| 2 | Gateway High School | A | Rayner | Spencer | Shelton | Washington | NT | Verified |
| 3 | Vashon High School | A | Cody | Murphy | Morris | Brown | 46.37 | Verified |
| 4 | Miller Career Academy | A | Woods | Draper | Walker | Little | 47.13 | Verified |
| 5 | Roosevelt High School | A | Williams | Williams | Dick | Milton | NT | Verified |
| 6 | Metro High School | A | Badillo | Long | Were | Penny-Johnson | NT | Verified |
