| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | KIPP St. Louis | A | Brown | Davis | Green | Hilliard | NT | Unverified: |
| 2 | Miller Career Academy | A | Price | Dorsey | Brooks-Cann | Jones | 56.69 | Verified |
| 3 | Gateway High School | A | Bass | Carothers | Nevils | Hampton | 54.11 | Verified |
| 4 | Metro High School | A | Upadhya | Couilloud | Clair | Tate | DQ | Verified |
| 5 | McKinley Classical Leadership Hi | A | Butler | Hunsberger | Stuckey | Solanke | 59.11 | Verified |
| 6 | Vashon High School | A | Amerson | Boyd | Mosely | Taylor | 1:00.23 | Verified |
