| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | McKinley Classical Leadership Hi | A | Cotton | Winfield | Brooks | Bledsoe | NT | Unverified: |
| 2 | Gateway High School | A | Calvin | Rayner | Bell | Warren | 1:41.56 | Verified |
| 3 | Roosevelt High School | A | Smith | Smalls | Murphy | Hill | 1:40.13 | Verified |
| 4 | Sumner High School | A | Abdi | Blair | Caldwell | Lacy | 1:40.55 | Verified |
| 5 | Carnahan High School | A | Holmes | Mull | Clark | Hester | 1:43.64 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Miller Career Academy | A | Forte | Gleghorn | Williams | Slayton | 1:38.77 | Verified |
| 2 | Soldan International Studies Hig | A | Washington | Clemons | Johnson | Nebsit | DQ | Verified |
| 3 | Lift for Life Academy Charter Hi | A | Morgan | Landers | Penn | Owens | 1:34.00 | Verified |
| 4 | KIPP St. Louis | A | Baker | Casimere | Cook | Moore | 1:36.27 | Verified |
| 5 | Vashon High School | A | Hill | Johnson | Morris | Smith | 1:38.44 | Verified |
| 6 | Metro High School | A | Carter | Fisher | Dawson | Marley | NT | Verified |
