| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Carnahan High School | A | Brothers | Long | Banks | Clark | NT | Unverified: |
| 2 | Soldan International Studies Hig | A | Washington | Clemons | Johnson | McRobert | NT | Unverified: |
| 3 | KIPP St. Louis | A | Davis | Ray | Ward | Aziz | NT | Unverified: |
| 4 | Roosevelt High School | A | Smith | Campbell | Murphy | Smalls | NT | Unverified: |
| 5 | Sumner High School | A | Jones | Perkins | Henry | Blair | NT | Unverified: |
| 6 | Gateway High School | A | Bell | Calvin | Quarles | Rayner | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Collegiate School of Medicine an | A | Robinson | Barket | Hicks | Bloch | 4:48.84 | Verified |
| 2 | Lift for Life Academy Charter Hi | A | Norrington | Penn | Owens | Landers | 4:28.31 | Verified |
| 3 | Vashon High School | A | Chandler | Morris | Cody | Morris | 1:45.60 | Unverified: |
| 4 | Miller Career Academy | A | Woods | Walker | Draper | Little | 4:18.26 | Verified |
| 5 | McKinley Classical Leadership Hi | A | Bledsoe | Flenoid | Johnson | Johnson | 4:46.38 | Verified |
| 6 | Metro High School | A | Baum-Scholle | Furman | Flenoid | Dawson | 4:53.71 | Verified |
