| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Soldan International Studies Hig | A | Greenlee | Sommerville | Adams | Johnson | NT | Unverified: |
| 2 | Collegiate School of Medicine an | A | VandenBrink | Taylor | Robinson | Redmond | NT | Unverified: |
| 3 | Vashon High School | A | Amerson | Taylor | Mosely | Boyd | NT | Unverified: |
| 4 | McKinley Classical Leadership Hi | A | Kamara | Hunsberger | Butler | Miller | NT | Unverified: |
| 5 | Metro High School | A | Jones | Fuchs | Kamara | Leonard | NT | Unverified: |
| 6 | Miller Career Academy | A | Price | Mosley | Davis | Brooks-Cann | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | KIPP St. Louis | A | Brown | Davis | Green | Hilliard | NT | Unverified: |
| 3 | Lift for Life Academy Charter Hi | A | Taylor | Lindsey | Fisher | Fowler | 5:46.58 | Verified |
| 4 | Sumner High School | A | Hill | Dupree | Tankins | Sombright-Dorsey | NT | Unverified: |
| 6 | Gateway High School | A | Bass | Carothers | Nevils | Hampton | NT | Unverified: |
