| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Carnahan High School | A | Holmes | Hester | Mull | Clark | 1:43.64 | Verified |
| 3 | Sumner High School | A | Blair | Abdi | Caldwell | Henry | 1:40.55 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Gateway High School | A | Bell | Calvin | Rayner | Warren | 1:37.02 | Verified |
| 2 | Vashon High School | A | Hill | Morris | Phillips | Smith | 1:35.82 | Verified |
| 3 | KIPP St. Louis | A | Brown | Davis | Ward | Wiley | 1:32.22 | Verified |
| 4 | Miller Career Academy | A | Forte | Gleghorn | Slayton | Williams | 1:35.50 | Verified |
| 5 | Soldan International Studies Hig | A | Washington | Coleman | Nebsit | Johnson | 1:36.78 | Verified |
