| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | KIPP St. Louis | A | Jones | Jones | Jones | Muhammad | 50.24 | Verified |
| 2 | Vashon High School | A | Brown | Morris | Cody | Dickerson | 47.60 | Unverified: PHL Relays |
| 3 | Miller Career Academy | A | Harlan | Little | Walker | Draper | 44.95 | Verified |
| 4 | Gateway High School | A | Dickerson | Harris | Zevulun | Shelton | 46.78 | Verified |
| 5 | Roosevelt High School | A | Williams | Williams | Dick | Milton | NT | Verified |
