| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | KIPP St. Louis | A | Brown | Davis | Green | Hilliard | 59.55 | Verified |
| 2 | Collegiate School of Medicine an | A | Redmond | Bohlen | Luster | Robinson | 55.92 | Verified |
| 3 | Metro High School | A | Green | Thompson | Upadhya | Fuchs | 54.19 | Verified |
| 4 | Miller Career Academy | A | Mosley | Jones | Dorsey | McClendon | 54.36 | Verified |
| 5 | Vashon High School | A | Arnold | Boyd | Mosely | Amerson | 58.47 | Verified |
