| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | DAWSON, Vincent | SR | Metro High School | 2:14.31 | Verified |
| 2 | CUSTIS, Andrew | JR | Metro High School | 2:21.84 | Verified |
| 3 | GROOMS, Brandon | JR | KIPP St. Louis | 2:24.96 | Verified |
| 6 | FLEMING, Shaun | JR | Soldan International Studies Hig | 2:39.87 | Verified |
| 7 | JONES, Anthony | SO | Collegiate School of Medicine an | 2:46.73 | Verified |
| 8 | MILTON, Kevion | 9 | Roosevelt High School | NT | Unverified: |
| 9 | BUCKNER, Jazzy | JR | Soldan International Studies Hig | NT | Unverified: |
| 10 | HARLAN, Kyree | SO | Miller Career Academy | NT | Unverified: |
| 11 | CHAPPLE-GRIFFIN, Jayson | JR | Roosevelt High School | NT | Unverified: |
| 12 | WASHINGTON, Juan | 9 | Gateway High School | NT | Unverified: |
