| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | St. Louis PAL | A | Holmes Iv | Estrada | Edgell | Hawthorne | NT | |
| 4 | ST Louis Scorpions Track Club | A | Phelps | Sherrod | Moore | Evans | NT | |
| 5 | Blues Track Club of Greater St | A | Jones | Williams | Booth Ii | Williams Iii | NT | |
| 6 | Ultimate Speed Academy | A | Freeman | Saldivar | Graham | Cross | NT |
