| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Ultimate Speed Academy | B | Shim | Shim | Langford | Moore | NT | |
| 2 | Blues Track Club of Greater St | A | Hensgens | Madison | Dickerson | Graham | NT | |
| 3 | ST Louis Scorpions Track Club | A | Jones | Hammond | Kerr | Whitaker | NT | |
| 4 | Thunder Speed Track Club | A | Mcmullen | Ford | Jennings | Combs | NT | |
| 5 | Blues Track Club of Greater St | B | Johnson | White | Moody | Clark | NT | |
| 6 | Tallawah Track and Field | A | Bennett | Spahic | Samuelson | Turntine | NT | |
| 7 | Ultimate Speed Academy | A | Rogers | Higgins | Norwood | Sanders | NT |
