| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Blues Track Club of Greater St | A | Hubbard | Salter | Robinson | James Jr | NT | |
| 2 | ST Louis Scorpions Track Club | A | Williams | Chatman | Fox | Walton Jr | NT | |
| 7 | Ultimate Speed Academy | A | Collins | Graham | Thornton | Thornton | NT |
