| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Ultimate Speed Academy | A | Smith | Moore | Lowe | Gasama | NT | |
| 2 | ST Louis Scorpions Track Club | C | Williams | Spencer | Curtis | Brown | DNS | |
| 3 | ST Louis Scorpions Track Club | B | Clay | Massey | Moore | Rayner | NT | |
| 4 | ST Louis Scorpions Track Club | D | Wills Jr | Griffin-Badoy | Smith | Allen | DNS | |
| 5 | Ultimate Speed Academy | B | Jackson | Jenkins | Ming | Jackson | NT | |
| 6 | Blues Track Club of Greater St | A | Bealer | Clark | Lane | Houston | NT | |
| 7 | ST Louis Scorpions Track Club | A | Archie | Courtney | Hayes | Settles | NT |
