| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | EARNST, Emerie | Blues Track Club of Greater St | NT | ||
| 2 | MCCLENDON, Imani | ST Louis Scorpions Track Club | NT | ||
| 3 | HARPER-MCCAIN, Kamora | ST Louis Scorpions Track Club | NT | ||
| 4 | LOVE, Jamaya | St. Louis PAL | NT | ||
| 5 | EVANS, Quintasia | Herbert Hoover | NT | ||
| 6 | SHELTON, Jamira | Thunder Speed Track Club | NT | ||
| 7 | TATE-PATTERSON, Kahmora | Blues Track Club of Greater St | NT | ||
| 8 | WARREN, Reese | Mustangs Track Team | NT |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | PRATT, Kimora | Blues Track Club of Greater St | NT | ||
| 2 | THOMPSON, Darrina | St. Louis Saints | NT | ||
| 3 | ANTHONY, Aryanna | Mustangs Track Team | NT | ||
| 4 | STAMPS, Ryann | Ultimate Speed Academy | 28.52 | ||
| 5 | JONES, Autumn | Herbert Hoover | 28.71 | ||
| 6 | BLUE, Olivia | Thunder Speed Track Club | NT | ||
| 7 | RAYNER, Joy | ST Louis Scorpions Track Club | NT | ||
| 8 | JONES, Kayla | ST Louis Scorpions Track Club | NT |
