| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 5 | ROBINSON, Kasen | Blues Track Club of Greater St | 2:33.71 | ||
| 6 | CROSS, Tristan | Ultimate Speed Academy | NT | ||
| 7 | HOLMES IV, Harry | St. Louis PAL | NT | ||
| 8 | SALDIVAR, Dominic | Ultimate Speed Academy | NT | ||
| 9 | LEMEN, Parker | Blues Track Club of Greater St | NT | ||
| 10 | DURANT, Brandon | St. Louis Saints | NT | ||
| 11 | ALLEN, Mark | ST Louis Scorpions Track Club | NT | ||
| 12 | JACKSON, Jeremiah | Herbert Hoover | NT | ||
| 13 | HAWTHORNE, Tyler | St. Louis PAL | NT | ||
| 14 | WASHINGTON-DIENG, Amadou | Mustangs Track Team | NT | ||
| 15 | POWELL, Evan | Thunder Speed Track Club | NT | ||
| 16 | DURANT, Braylon | St. Louis Saints | NT | ||
| 17 | SPRATT, Devin | Herbert Hoover | NT | ||
| 18 | UNION, Charles | ST Louis Scorpions Track Club | NT | ||
| 19 | ESTRADA, Sam | St. Louis PAL | NT | ||
| 20 | THORNTON III, Don | St. Louis Saints | NT | ||
| 21 | MILLER, Jackson | Unattached | NT |
