| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | SETTLES III, Geno | ST Louis Scorpions Track Club | NT | ||
| 2 | EDWARDS, Gideon | R.C Striders | NT | ||
| 3 | AARON, Titan | Ultimate Speed Academy | NT | ||
| 4 | FISCHER, Daniel | Missouri Elite Sports Club | NT | ||
| 5 | STEPHEN, Noah | Ultimate Speed Academy | NT | ||
| 6 | LITTLEJOHN, Michael | Herbert Hoover | NT | ||
| 7 | SPARKMAN, Elijah | St. Louis Saints | NT | ||
| 8 | WESLEY, Laden | Herbert Hoover | NT | ||
| 9 | SPAHIC, Amar | Tallawah Track and Field | NT | ||
| 10 | MYERS JR., Jermaine | Mustangs Track Team | NT | ||
| 11 | THOMPSON, Jayceon | ST Louis Scorpions Track Club | NT | ||
| 12 | MOORE, Michael | St.Charles Heat | NT | ||
| 13 | REEVES, Jaxson | Ultimate Speed Academy | NT | ||
| 14 | BETTS, Jaydan | Ultimate Speed Academy | NT | ||
| 15 | THORNTON, Adonis | St. Louis Saints | NT | ||
| 16 | NEELY, Alexander | Dashers Track and Sports | NT | ||
| 17 | FARRAR JR, Christopher | U. City Xplosion Track & Field | NT | ||
| 18 | HARKLESS JR, Lonnell | ST Louis Scorpions Track Club | NT |
