| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CARLSON, Cylie | JR | Ste. Genevieve High School | 6:04.31 | Verified |
| 2 | LIN, CHLOE | SO | Sikeston High School | 6:15.41 | Verified |
| 3 | CALLAWAY, Aliya | SR | Delta High School | 6:16.94 | Verified |
| 4 | MERRICK, Payton | JR | Kelly High School | 6:44.47 | Verified |
| 5 | FITZGERALD, MORGAN | JR | Sikeston High School | 6:49.20 | Verified |
| 6 | DEHNE, Eden | JR | Saxony Lutheran High School | 6:58.65 | Verified |
| 7 | BROWN, Rylie | 9 | Malden High School | 7:25.00 | Unverified: |
| 8 | FLESHMAN, Elizabeth | 9 | Fox High School | NT | Unverified: |
| 9 | YOCHIM, Ayelyn | 9 | Ste. Genevieve High School | NT | Unverified: |
| 10 | TEJEDA, Christi | SO | Kennett High School | NT | Unverified: |
| 11 | GOSCHE, Brooklyn | 9 | Notre Dame (Cape Girardeau) High | NT | Unverified: |
| 12 | HALE, Kali | 9 | Dexter High School | NT | Unverified: |
| 13 | BRITT, Chariti | 9 | Delta High School | NT | Unverified: |
| 14 | ROTH, Myra | 9 | Saxony Lutheran High School | NT | Unverified: |
| 15 | MARRIOTT, Emily | 9 | Central (Cape Girardeau) High Sc | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MARCUM, Isabella | JR | Fox High School | 5:14.11 | Verified |
| 2 | VOGELSANG, Kayla | SR | DeSoto High School | 5:21.99 | Verified |
| 3 | BROWN, Gabrielle | SR | Dexter High School | 5:22.88 | Verified |
| 4 | THIER, Ella | JR | Rockwood Summit High School | 5:29.85 | Verified |
| 5 | FAERBER, Allison | SR | Festus High School | 5:32.71 | Verified |
| 6 | CALDWELL, Lindsey | JR | Central (Cape Girardeau) High Sc | 5:39.30 | Verified |
| 7 | PIGG, JAILEY | SO | DeSoto High School | 5:41.84 | Verified |
| 8 | MARTIN, Reese | 9 | Rockwood Summit High School | 5:45.00 | Unverified: |
| 9 | EVANS, Katelyn | JR | Kelly High School | 5:47.88 | Verified |
| 10 | POPP, London | JR | Jackson High School | 5:49.00 | Unverified: |
| 11 | TURNER, Molly | JR | Notre Dame (Cape Girardeau) High | 5:53.75 | Verified |
| 12 | LEFTWICH, Ava | JR | Festus High School | 5:55.55 | Verified |
| 13 | WILSON, Mara | JR | Jackson High School | 5:57.00 | Unverified: |
| 14 | HEEB, Lani | JR | Kennett High School | 5:58.57 | Verified |
| 15 | SCHIWITZ, Logan | SO | Scott City High School | 6:01.50 | Verified |
