| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Central (Cape Girardeau) High Sc | A | NT | |||||
| 2 | Northwest (Cedar Hill) High Scho | A | NT | |||||
| 3 | Sikeston High School | A | NT | |||||
| 4 | Charleston High School | A | NT | |||||
| 5 | Saxony Lutheran High School | A | NT | |||||
| 6 | Kelly High School | A | NT | |||||
| 7 | Notre Dame (Cape Girardeau) High | A | NT | |||||
| 8 | Rockwood Summit High School | A | NT |
