| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | STAPLE, Christiana | SO | Sikeston High School | 3:18.59 | Verified |
| 2 | DEHNE, Eden | JR | Saxony Lutheran High School | 3:19.96 | Verified |
| 3 | MEYERS, Abigail | SO | Central (Cape Girardeau) High Sc | 3:20.16 | Verified |
| 4 | CALLAWAY, Aliya | SR | Delta High School | 3:21.00 | Verified |
| 5 | LIN, LUCY | SO | Sikeston High School | 3:41.60 | Verified |
| 6 | GOSCHE, Brooklyn | 9 | Notre Dame (Cape Girardeau) High | NT | Unverified: |
| 7 | BRITT, Chariti | 9 | Delta High School | NT | Unverified: |
| 8 | CALDWELL, Aimee | 9 | Central (Cape Girardeau) High Sc | NT | Unverified: |
| 9 | GREMINGER, Sadie | 9 | Ste. Genevieve High School | NT | Unverified: |
| 10 | HALE, Kali | 9 | Dexter High School | NT | Unverified: |
| 11 | MOORE, Abigail | SR | Ste. Genevieve High School | NT | Unverified: |
| 12 | HILLIN, Maggie | 9 | Saxony Lutheran High School | NT | Unverified: |
| 13 | GLASSER, Grace | JR | Scott City High School | NT | Unverified: |
| 14 | ESSNER, Gwendolyn | 9 | Kelly High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | FLIEGE, Layla | SR | Jackson High School | 2:22.00 | Unverified: |
| 2 | BROWN, Gabrielle | SR | Dexter High School | 2:24.07 | Verified |
| 3 | BROWN, Mia | SO | Rockwood Summit High School | 2:31.02 | Verified |
| 4 | HEEB, Lani | JR | Kennett High School | 2:35.68 | Verified |
| 5 | KURTZ, Grace | SO | Jackson High School | 2:37.00 | Unverified: |
| 6 | ROSSOMANNO, Caterina | 9 | Rockwood Summit High School | 2:40.00 | Unverified: |
| 7 | TURNER, Molly | JR | Notre Dame (Cape Girardeau) High | 2:40.96 | Verified |
| 8 | SCHIWITZ, Logan | SO | Scott City High School | 2:43.00 | Verified |
| 9 | MASON, Lauryn | SR | Fox High School | 2:45.67 | Verified |
| 10 | BARNETT, Livia | SO | Festus High School | 2:46.43 | Verified |
| 11 | SCHNABLE, Abigail | SO | Festus High School | 2:49.04 | Verified |
| 12 | PATTENGILL, Elly | SR | Kelly High School | 2:54.00 | Verified |
| 13 | BROWN, Rylie | 9 | Malden High School | 2:55.00 | Unverified: |
| 14 | LAURINE, Elizabeth | SO | Fox High School | 3:17.29 | Verified |
| 15 | YOUNT, Allison | SR | Kennett High School | 3:18.54 | Verified |
