| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | OVERBEY, Sadie | 9 | Kennett High School | NT | Unverified: |
| 2 | DAVIS, Hailie | 9 | Zalma High School | NT | Unverified: |
| 4 | MOORE, Janaye | SO | Sikeston High School | NT | Unverified: |
| 5 | HUCK, Kayden | 9 | Ste. Genevieve High School | NT | Unverified: |
| 7 | KIMBLE, Treasure | 9 | Charleston High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | METZGER, Gracie | JR | Jackson High School | 52.26 | Unverified: |
| 3 | DOCKERY, Taler | SO | Central (Cape Girardeau) High Sc | 50.20 | Verified |
| 4 | HAMILTON, Laticia | SR | Caruthersville High School | 47.75 | Verified |
| 5 | TURNBO, Allison | SO | Dexter High School | 50.13 | Verified |
| 6 | THOMAS, Madison | SR | Central (Cape Girardeau) High Sc | 52.20 | Verified |
| 7 | HALE, Bryanna | SR | Saxony Lutheran High School | 58.06 | Verified |
| 8 | HOWARD, AUTUMN | 9 | Jackson High School | 1:00.00 | Unverified: |
