| PL | Team | Squad | Time | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 |
|---|---|---|---|---|---|---|---|
| 1 | Rockwood Summit High School | A | 49.34 | ||||
| 2 | Notre Dame (Cape Girardeau) High | A | 51.16 | ||||
| 3 | Central (Cape Girardeau) High Sc | A | 52.81 | ||||
| 4 | Kelly High School | A | 54.44 | ||||
| 5 | Sikeston High School | A | 56.67 | ||||
| 6 | Saxony Lutheran High School | A | 1:00.16 | ||||
| 7 | Northwest (Cedar Hill) High Scho | A | 1:14.57 | ||||
| Charleston High School | A | NT |
